DSpace Coleção:

Neoplasias em 240 necrópsias de pacientes HIV positivos, realizados no Hospital Escola da Faculdade de Medicina da Universidade Federal do Triângulo Mineiro.

2007-05-04T00:00:00Z

Título: Neoplasias em 240 necrópsias de pacientes HIV positivos, realizados no Hospital Escola da Faculdade de Medicina da Universidade Federal do Triângulo Mineiro. Tipo: Tese

Diminuição da expressão renal da enzima conversora de angiotensina por meio de um pequeno RNA interferente reduz pressão arterial sistêmica em ratos espontaneamente hipertensos.

2006-06-27T00:00:00Z

Título: Diminuição da expressão renal da enzima conversora de angiotensina por meio de um pequeno RNA interferente reduz pressão arterial sistêmica em ratos espontaneamente hipertensos. Abstract: In the present study we evaluated the effects of a small interfering RNA (siRNA) targeting angiotensin converting enzyme (ACE) gene in spontaneously hypertensive rats (SHR). Nineteen male sixteen weeks-old SHR were submitted under anesthesia to femoral vessels catheterization for chronic recordings of arterial pressure (AP) and drug administration, respectively. After baseline AP and heart rate (HR) recordings in a computerized system, nine animals received by intravenous infusion 150ug/Kg of ACE-siRNA and other ten animals (control group) received also intravenously siRNA targeting enhanced green fluorescent protein (eGFP) gene, not expressed in mammalians. Following, each twelve hours for the next four days, continuous recordings of AP and HR for 30 minutes each one were performed in quiet and controlled room. At the end of forth day, after last AP recording and evaluation of angiotensin I (100 ng/Kg, i.v.) pressor response, all animals were sacrificed and lungs and kidneys were extracted to perform western-blot and RTPCR analysis of tissue ACE expression. Baseline values of mean AP (MAP) and HR did not differ between groups before treatment (182±3 mmHg e 366±10 bpm in ACEsiRNA group and 172±4 mmHg e 356±16 bpm in control group, respectively, p=ns). MAP values remained similar in both groups until second day, after which they began to decrease in ACE-siRNA group reaching the lowest values in the forth day (136±8 mmHg versus 167±4 mmHg in control, p<0,02). HR values as well as angiotensin I pressure responses did not differ between groups. Western-blot analysis of tissue ACE expression revealed 52,9% of reduction of kidneys ACE expression in ACEsiRNA group as compared to control group, without changes in lung ACE expression. Despite of reduction of protein expression in the kidneys of the treated animals, RTPCR was not able to demonstrate any change in messenger RNA expression for ACE in treated animals. These data suggest that a naked small RNA targeting ACE gene, intravenously injected, may act in vivo attenuating ACE expression in a tissueselective way, with marked repercussion on systemic arterial pressure in SHR. Since the expression of messenger RNA for ACE was not modified, a possible microRNA effect could be suggested. These findings point out small interferent RNAs or microRNAs as a promising strategy to treat systemic arterial hypertension. Tipo: Dissertação

Efeito da dieta hiperlipídico-protéica no metabolismo de ratos wistar adultos

2008-12-08T00:00:00Z

Título: Efeito da dieta hiperlipídico-protéica no metabolismo de ratos wistar adultos Abstract: The consumption of diets with low carbohydrate and high protein and fat is part of the list of diets used for weight loss. In principle, these diets lead to the mobilization of triglycerides to realize production of ketone bodies and maintenance of body functions. The present study tested the effect of a hyperlipidic and hyperproteic diet (HLP) with low carbohydrate content on body weight, weight of organs, food intake, serum biochemical parameters and pathological changes in liver of rats. We used 24 animals of which 12 for the group C and 12 to Experimental group, weighing on average 163 grams at the beginning of the experiment. Parameters such as: body weight, food intake, urine volume and pH, were performed weekly. The biochemical tests and the weight of the organs were carried out at the end of the experiment. We found that experimental animals gained more weight, accumulated more fat, than animals fed with balanced diet for rats, moreover they also showed a reduction in the size of the testicles and brain, suggesting that the composition of the diet may affect the metabolism. Organs such as liver, kidneys, spleen and have not changed. Independent of diet used, the animals did not alter the pattern of caloric intake. The experimental diet induced a significant increase in urine volume and a small reduction in urine pH. The experimental group developed hyperglycemia, hypertriglyceridemia, increasing of the HDL fraction of cholesterol and increase of the creatinine when compared with control. Among the hystopathological changes, there is the occurrence of steatosis in experimental group, showing the effect of the diets (HLP) in metabolism. Together, the results show that diets low in carbohydrates and high in fat and protein can cause various metabolic changes. Tipo: Dissertação

Aspectos clínicos-epidemiológicos e percepção de dor na anemia falsiforme.

2009-06-30T00:00:00Z

Título: Aspectos clínicos-epidemiológicos e percepção de dor na anemia falsiforme. Abstract: Sickle Cell Disease (SCD) is one of the main and most frequent genetic illnesses that attack human beings. It is characterized by the predominance of S hemoglobin (HbS) with clear clinical manifestations: sickle cell anemia (HbSS) and associations with other variants of hemoglobin: HbC, HbD and thalassemias (a and b). It is predominant among black and dark-skinned people. There is an estimate of more than 7,200,000 people with the sickle cell trait in Brazil, and 3,500 children are born yearly with SCD. Erythrocytes with S hemoglobin, under conditions of hypoxia, take on the shape of a sickle, with the possibility of leading to a total occlusion of the capillaries, causing acute tissue lesions, with pain crisis and chronic lesion of organs. Illnesses, exposure to cold and stress are factors that can trigger the crisis. Pain due to the crisis of vein-occlusion is the major cause of hospitalization and is associated to early death. By means of home visits, interviews were carried out with SCD, collecting clinical-epidemiologic and pain data. The patients lived in Uberaba, and were 18 or more years old. Among the 47 sample patients there were: 78% black; 17% dark-skinned; 4.3% white; 59.6% females. Type of hemoglobinopathy: 63.8% SS; 17% SC; 10.6% Sb thalassemias; 78.7% have already had blood transfusions. Pain crisis: frequent crisis in 85.1%, with an average of 7.02 crisis per year. Measurement of pain: END average 5.5 (moderate pain); MPQ 35.9. Most frequent places: lower limbs 74.5%, shoulders and upper limbs 57.4%, lumbar and sacral spine, coccyx 53.2%. Predominant season: winter (68.1%). Feelings in relation to the illness: acceptance 38.3%, revolt 21.3%, sadness 17%. Changes after diagnosis: 46.8% get worse in relation to work, 31.9% in relation to leisure. It is possible to conclude that the illness affects the person at all levels and the pain can be totally incapacitating for many of the patients with SCD. Tipo: Dissertação