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| Campo DC | Valor | Idioma |
|---|---|---|
| dc.creator | SILVA, Isabela Rios da | - |
| dc.creator.ID | 08946968664 | pt_BR |
| dc.creator.Lattes | http://lattes.cnpq.br/8719529622576108 | pt_BR |
| dc.contributor.advisor1 | RODRIGUES, Denise Bertulucci Rocha | - |
| dc.contributor.advisor1ID | 10676897886 | pt_BR |
| dc.contributor.advisor1Lattes | http://lattes.cnpq.br/5953745136489913 | pt_BR |
| dc.contributor.advisor-co1 | RODRIGUES JUNIOR, Virmondes | - |
| dc.contributor.advisor-co1ID | 45813493620 | pt_BR |
| dc.contributor.advisor-co1Lattes | http://lattes.cnpq.br/8909243237236516 | pt_BR |
| dc.date.accessioned | 2022-07-25T17:28:53Z | - |
| dc.date.available | 2017-02-10 | - |
| dc.date.available | 2022-07-25T17:28:53Z | - |
| dc.date.issued | 2017-02-10 | - |
| dc.identifier.citation | SILVA, Isabela Rios da. Avaliação morfométrica do colágeno total, tipo I e tipo III e análise da expressão in situ de citocinas em queloides. 2017. 69f . Dissertação (Mestrado e Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2017 . | pt_BR |
| dc.identifier.uri | http://bdtd.uftm.edu.br/handle/123456789/1343 | - |
| dc.description.resumo | Os queloides são resultantes de alterações fisiológicas no processo cicatricial. São caracterizados pelo crescimento além das bordas da lesão inicial e pela não regressão espontânea. Seus sinais e sintomas incluem dor, prurido, rubor e enrijecimento local e pode causar desconfortos psicológicos, devido à desfiguração estética muito comum nestas lesões. Caracteriza-se pela deposição excessiva de colágeno e diversas citocinas podem estar contribuindo na fisiopatologia. O objetivo deste estudo foi avaliar através de morfometria as fibras colágenas e analisar in situ a expressão das citocinas em lesões de queloides e compará-las ao grupo controle. Um total de 33 pacientes foram acompanhados no ambulatório da cirurgia plástica, tratados com triancinolona 20mg/ml intralesional e encaminhados para excisão da lesão. Os fragmentos foram armazenados em RNA later e em formol tamponado. O grupo controle constituiu-se de 40 fragmentos de cicatriz normal retiradas, em sua maioria, de pacientes secundíparas ou multíparas durante cesariana. Para a captura de imagens e quantificação das fibras colágenas foram utilizados o sistema Leica Qwin Plus® e Image J®. Os resultados demonstraram uma predominância do sexo feminino (60,60%), etnia não branca (60,60) e descendência negra direta (66,66%). Os queloides apresentaram aumento significativo no colágeno total e tipo III (p<0,0001; p=0,0001). Significativamente, a expressão de mRNA para o TGF-β nos queloides apresentou-se aumentada, a de IFN-γ, IFN-γR1 e IL-10 foram menores, sem diferença estatística para o IFN-γR1 e o TNF-α não apresentou diferença significativa (p<0,001; p=0,009; p=0,246; p=0,037; p=0,911) comparados ao grupo controle. Correlações entre colágeno tipo III a expressão de mRNA do TGF-β foram positivas e significativas (p=0,001, z= 3,210), do IFN-γ, IFN-γR1 e IL-10 negativa e significativa (p=0,015, z=-2,425; p=0,021, z=-2,303; p=0,014, z=-2,445) e do TNF-α sem diferença estatística (p=0,555, z=0,590). Dessa forma, com estes resultados, pode-se sugerir que as citocinas pró-infamatórias e anti-inflamatórias podem estar auxiliando diretamente na patogênese do queloide principalmente ao induzir uma cicatrização mais imatura, com síntese excessiva de colágeno e com contração cicatricial ineficiente. Além disso, o TGF-β parece ser o maior fator de indução da ativação e proliferação dos fibroblastos, sendo um alvo importante de estudos para elucidar a fisiopatologia do queloide. | pt_BR |
| dc.description.abstract | Keloids are the result of physiological changes in the healing process. Characterized by growth beyond the borders of the initial lesion and by non-spontaneous regression. Its signs and symptoms include pain, itching, flushing and local stiffness and can cause psychological discomforts due to the aesthetic disfigurement very common in these lesions. It is characterized by the excessive deposition of collagen and several cytokines may be contributing to the pathophysiology. The objective of this study was to evaluate the collagen fibers, analyze in situ expression of cytokines in keloid lesions, and compare to the control group. 33 patients were followed up at the plastic surgery outpatient, treated with triamcinolone 20 mg/ml and referred for excision of the lesion. The fragments were stored in later RNA and buffered formalin. The control group consisted of 40 normal scar fragments taken from secondary or multiparous patients during cesarean section. The Leica Qwin Plus® and Image J® system were used for image capture and quantification of the collagen fibers. The results showed a predominance of women (60.60%), non-white (60.60) and direct black ancestry (66.66%). Keloid showed a significant increase in total and type III collagen (p <0.0001, p = 0.0001). Significantly, the expression of mRNA for TGF-β in keloid was increased, the expression of IFN-γ, IFN-γR1 and IL-10 were lower, and with no statistical difference for IFN-γR1 and TNF-α showed no statistical difference (p<0.001, p=0.009, p=0.246, p=0.037; p=0.911) compared to the control group. Correlations between collagen type III and TGF-β mRNA expression were positive and significant (p=0.001, z=3.210), and IFN-γ, IFN-γR1 and IL-10, were negative and significant (p=0.015, z=-2.425, p=0.021, z=-2.303, p=0.014, z=-2.445) and TNF-α with no statistical difference (p=0.555, z=0.590). Thus, with these results, it can be suggested that pro-inflammatory and anti-inflammatory cytokines may be directly assisting in the pathogenesis of the keloid mainly by inducing a more immature healing, with excessive collagen synthesis and with inefficient wound contraction. In addition, TGF-β appears to be the major induction factor for fibroblast activation and proliferation, and is an important target for studies to elucidate the keloid pathophysiology. | pt_BR |
| dc.description.sponsorship | Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq | pt_BR |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES | pt_BR |
| dc.description.sponsorship | Fundação de Ensino e Pesquisa de Uberaba - FUNEPU | pt_BR |
| dc.description.sponsorship | Fundação de Amparo a Pesquisa do Estado de Minas Gerais - FAPEMIG | pt_BR |
| dc.description.sponsorship | Universidade Federal do Triângulo Mineiro - UFTM | pt_BR |
| dc.format | application/pdf | * |
| dc.language | por | pt_BR |
| dc.publisher | Universidade Federal do Triângulo Mineiro | pt_BR |
| dc.publisher.department | Instituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da Saúde | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.initials | UFTM | pt_BR |
| dc.publisher.program | Programa de Pós-Graduação em Ciências da Saúde | pt_BR |
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ZHOU, R. et al. miR-21 promotes collagen production in keloid via Smad7. Burns, Oct 4 2016. | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
| dc.subject | Queloide. | pt_BR |
| dc.subject | Colágeno. | pt_BR |
| dc.subject | Citocinas. | pt_BR |
| dc.subject | keloid. | pt_BR |
| dc.subject | Collagen. | pt_BR |
| dc.subject | Cytokines. | pt_BR |
| dc.subject.cnpq | CNPQ::CIENCIAS DA SAUDE::MEDICINA | pt_BR |
| dc.title | Avaliação morfométrica do colágeno total, tipo I e tipo III e análise da expressão in situ de citocinas em queloides | pt_BR |
| dc.type | Dissertação | pt_BR |
| Aparece nas coleções: | Programa de Pós-Graduação em Ciências da Saúde | |
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