Imunomarcação epitelial e estromal de citocinas em neoplasias de ovário

Abstract

Objectives: The aims of this study were to determine whether there are differences in tissue stromal and epithelial staining by immunohistochemistry of cytokines (IL2, IL8, TNF-R1, IL-5, IL-6 and IL-10) between benign tumors and malignant primary ovarian tumors, and to evaluate the relationship of stromal and epithelial staining in ovarian cancer with clinical and pathological prognostic factors. Material and Methods: We evaluated prospectively 56 patients underwent surgical treatment for ovarian cysts in accordance with pre-established criteria and with confirmed diagnosis of ovarian neoplasm. Immunohistochemistry study for cytokines (IL2, IL5, IL6, IL8, IL10, TNF-alpha) was performed. The prognostic factors were evaluated using the Fisher’s exact test. The significance level was less than 0.05. Results: We evaluated 56 patients with ovarian neoplasm (28 malignant and 28 benign neoplasms). The immunohistochemical staining by TNF (stroma and epithelium), IL5 (stroma and epithelium) and IL10 (only stroma) were stronger in benign neoplasms. IL2 immunostaining was stronger in malignant tumors compared with benign tumors. IL8 immunostaining was weaker in the stroma of benign neoplasms compared with ovarian cancer. Regarding IL-6 immunostaining, there was no statistical significance. In evaluating of stromal IL2, the histological grade 3 was significantly correlated with immunohistochemical of score 2/3 (p=0.003). TNF-alpha immunohistochemical staining scores 2 or 3 was related to disease-free survival ≤ to 30 months, suggesting that it is a factor of poor prognosis (p=0.017). There was no statistical significance in relation at other cytokines studied, both in the evaluation of the stroma as the epithelium.