Avaliação de fatores inibitórios desencadeados por células T reguladoras sobre linfócitos citotóxicos em pacientes com neoplasias linfoproliferativas

Abstract

Chronic lymphoproliferative neoplasm are diseases characterized by monoclonal expansion and accumulation of apparently mature lymphocytes which have a proliferative and/or survival advantage in bone marrow, peripheral blood and lymph nodes. The immunological mechanisms involved in cell induction and inactivation are complex and still poorly understood in these neoplasms. Therefore, this study aimed to evaluate the gene expression of TGFβ, PD1, PDL1, CTLA-4, FoxP3, PRF1 and GZMB in patients with nonHodgkin’s lymphoma (NHL) and chronic lymphocytic leukemia (CLL) attended at Hospital of Clinics of Federal University of Triângulo Mineiro (UFTM). This study comprehended twenty patients with NHL, 14 with CLL and 12 healthy individuals. Relative quantification of genes was perfomed by qPCR. Non-Hodgkin lymphoma patients, showed a significantly lower expression of TGFβ when compared to the control group (p=0,041). The same was not observed in relation to the other genes. Non-Hodgkin lymphoma patients, when characterized by severity, showed a higher expression of PDL1 (p=0,049) in individuals with aggressive NHL. Regarding the outcome, NHL patients who died had significantly lower levels of PRF1 expression than those who remained alive (p=0,0474). When analysed chronic lymphocytic leukemia (CLL) patients, no statistically significant difference in any of the genes evaluated. The data found here can contribute to new studies about the genes evaluated, as well as new markers with prognostic value. The understanding of tumor immunology combined with the evaluation of gene expression contributes to a better understanding of the immune response pattern.