Processos patológicos da lâmina própria sob hipóxia observados na língua de natimortos autopsiados

Abstract

Oxygen sensing is a property of all tissues, and the response to hypoxia is multidimensional, involving intracellular networks related to the transduction of hypoxiainduced responses. The most important stress to which the fetus and newborn are clinically subject is hypoxia, as during pregnancy it has a direct impact on fetal development. The objective of the study was to analyze, through morphometric analysis, the thickness of the lamina propria in the tongues of autopsied fetuses at different gestational ages, weight, tongue formation and its behavior in response to hypoxia and chronic complications. Tongues (n = 53) were collected from autopsied fetuses at 23-40 weeks of gestational age and were macroscopically tested for length and width measurements. Fifty-one tongue fragments were collected and processed histologically. Slides were stained with hematoxylin and eosin and immunostained with anti-HIF-1α antibody. The evaluation of the thickness of the lamina propria, presence of papillae and immunostaining were performed using a common light microscope. It was observed positively and significantly between lamina propria thickness and gestational age, as well as between epithelium thickness and mean tongue thickness and length. There was a positive and significant difference between epithelium thickness and fetal weight, and comparing the HIF positive and negative groups, there was a significant increase in lamina propria thickness in the HIF positive group. The dynamic between vascular density and mean tongue length and width was negative and significant. Vascular density in relation to the lamina propria, fetal weight and gestational age had a positive but not significant adaptation. Advancing gestational age is linked to the development of lamina propria thickness, tongue growth, fetal weight, and papilla formation. Faced with the pathological process of hypoxia, there was an increase in lamina própria thickness, generating the hypothesis of reactive tissue to fetal stress. Hypoxia is a process involved in different pregnancy disorders and complications in the development of fetal health.