Análise da citotoxicidade e imunomodulação de biovidro puro e nanoestruturados com TiO2

Abstract

The incorporation of nanocrystals into the molecular structure of bioglasses is innovative, which allows the intensification of various physical, chemical and biological properties. These properties need to be better characterized in various clinical applications. Therefore, our research group developed a new synthesis method, with the incorporation of Titanium Dioxide (TiO2) nanocrystals into the molecular structure of bioglasses, in the SNCP glass system (SiO2-Na2O-CaO-P2O5). The study was designed with the aim of starting the process of analyzing the potential clinical application of these new biocompounds. Therefore, the objective of the study was to evaluate the cytotoxicity and immunomodulation of new nanostructured bioglasses with TiO2, through viability in human blood cells (PBMC), cytokine measurement and survival assay in Galleria mellonella larvae. The results indicate that pure and nanostructured bioglass treatments lead to increased secretion of IFN-γ, IL-1β, and IL-6 by peripheral blood mononuclear cells (PBMCs), while TNF- α secretion is reduced. Cytotoxicity assays revealed that pure bioglass and nanostructured bioglass containing 10% titanium (Ti) exhibited lower cytotoxicity compared to formulations containing 20%, and 30% Ti in PBMCs. However, in vivo experiments using the Galleria mellonella larvae model demonstrated no toxicity even at higher concentrations (100 μg/larvae). These findings suggest that incorporating TiO2 into SNCP bioglass can improve its biological properties.