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http://bdtd.uftm.edu.br/handle/123456789/2039Registro completo de metadados
| Campo DC | Valor | Idioma |
|---|---|---|
| dc.creator | MARTINS, Lívia Alves | - |
| dc.creator.ID | 08947011690 | pt_BR |
| dc.creator.Lattes | http://lattes.cnpq.br/8964579477820145 | pt_BR |
| dc.contributor.advisor1 | RODRIGUES, Aldo Rogelis Aquiles | - |
| dc.contributor.advisor1ID | 83546448634 | pt_BR |
| dc.contributor.advisor1Lattes | http://lattes.cnpq.br/6645292290768657 | pt_BR |
| dc.date.accessioned | 2025-09-09T16:08:12Z | - |
| dc.date.available | 2014-12-12 | - |
| dc.date.available | 2025-09-09T16:08:12Z | - |
| dc.date.issued | 2014-12-12 | - |
| dc.identifier.citation | MARTINS, Lívia Alves. Imunolocalização de canais para sódio voltagem-dependentes em neurônios dos núcleos ambíguo e dorsal motor do vago. 2014. 75f . Dissertação (Mestrado em Ciências Fisiológicas) - Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Triângulo Mineiro, Uberaba, 2014 . | pt_BR |
| dc.identifier.uri | http://bdtd.uftm.edu.br/handle/123456789/2039 | - |
| dc.description.resumo | O núcleo ambíguo (NA) é uma coleção de neurônios motores distribuídos ventrolateralmente na formação reticular do bulbo que projetam fibras eferentes viscerais especiais para faringe, laringe, esôfago e via fibras eferentes viscerais gerais para pulmão e coração. O núcleo dorsal motor do vago (DMV) contém neurônios autonômicos situados dorsomedialmente no bulbo que enviam fibras viscerais gerais principalmente para as vísceras abdominais como estômago, fígado, pâncreas e intestino. O objetivo desse estudo foi avaliar a expressão de canais para sódio dependentes de voltagem (Nav) e sua localização subcelular nos neurônios das diferentes formações do NA e no DMV. Ratos Wistar de 30 dias foram perfundidos transcardiacamente e reações de imunofluorescência de dupla ou tripla marcação foram realizadas em fatias coronais de 25 a 40 m do bulbo. A identificação do NA e suas formações foi feita através de marcação anti-colina acetiltransferase (ChAT). Experimentos com o traçador retrógrado toxina colérica conjugada ao Alexa Fluor 488 (CTb) injetado no parênquima pulmonar ou a expressão do marcador de lesão neuronal ATF-3, após a ressecção do nervo laríngeo recorrente (RLN), foram também usados para identificação de neurônios no NA. Neurônios da formação loose (NAl, n=8), semicompacta (NAsc, n=8) e compacta (NAc, n=168) apresentaram maior diâmetro de 33 ± 1,6; 36 ± 0,4 e 22 ± 0,3 m, e ASS (área de silhueta somática) média de 462 ± 39,6; 503 ± 28,7 e 248 ± 7,3 m2 , respectivamente. Na formação NAc foi observada ampla heterogeneidade de neurônios. O traçador CTb identificou neurônios autonômicos da NAc que apresentaram marcação anti-Nav1.7 somática e em prováveis segmentos iniciais do axônio (AIS). Foi observada expressão de Nav1.6 em AIS de neurônios das NAc, NAsc e NAl, e tanto na NAl quanto na NAc foi observada correlação positiva entre a ASS e a extensão de marcação anti-Nav1.6 no AIS. Entre os neurônios Nav1.6 positivos, estão incluídos os motoneurônios que inervam a laringe, visto que também expressaram o fator ATF-3 após a ressecção do RLN. Foi observada também a expressão do canal Nav1.2 em prováveis dendritos de neurônios situados na NAc. Nos neurônios do DMV foi observada apenas a expressão do canal Nav1.7 no soma e possíveis AIS. Os resultados sugerem que neurônios motores do NA que inervam músculos branquioméricos utilizam o canal Nav1.6 para geração do potencial de ação, enquanto neurônios motores autonômicos parecem depender da atividade do canal Nav1.7. | pt_BR |
| dc.description.abstract | The nucleus ambiguus (NA) is a collection of neurons distributed ventrolaterally in the reticular formation of medulla that projects through special visceral efferent fibers to pharynx, larynx, esophagus or through general visceral efferent fibers to lung and heart. Dorsal motor nucleus of the vagus (DMV) contains autonomic neurons located dorsomedially in the medulla that send general visceral fibers mainly to abdominal viscera such as the stomach, liver, pancreas and intestines. The aim of this study was to evaluate the expression of voltage-gated sodium channels (Nav) and their subcellular location in neurons of different formations of NA and DMV. Wistar rats of 30 days were perfused transcardially and double or triple immunofluorescence staining was performed in 25 to 40 m coronal section of medulla. The identification of the NA formations was made through the analysis of immunofluorescence against choline acetyltransferase (ChAT). Experiments with retrograde tracer cholera toxin conjugated to Alexa Fluor 488 (CTb) applied to the lung parenchyma or the expression of neuronal injury marker ATF-3, after recurrent laryngeal nerve resection (RLN), were also used to identify neurons in the NA. Neurons in the loose formation (NAl, n=8), semi-compact (NAsc, n=8) and compact (NAc, n=168) had major diameter of 33 ± 1.6; 36 ± 0.4 and 22 ± 0.3 m, and somatic silhouette area (SSA) average of 462 ± 39.6; 503 ± 28.7 and 248 ± 7.3 m2 , respectively. Within NAc formation was observed extensive heterogeneity of neurons. The retrograde tracer CTb identified autonomic neurons in the NAc that showed anti-Nav1.7 staining at soma and presumed axon initial segments (AIS). Nav1.6 expression was observed in AIS of neurons within NAc, NAsc and NAl, and both NAL and NAc showed positive correlation between SSA and the extent of anti-Nav1.6 labeling along the AIS. Among the Nav1.6 positive neurons those that innervate the larynx are included, based on ATF-3 expression after resection of RLN. We also observed Nav1.2 staining in presumed dendrites of NAc neurons. At the neurons of the DMV an anti-Nav1.7 staining was observed at the soma and AIS. The results suggest that motoneurons of NA that innervate branchiomeric muscles use Nav1.6 channel isoform to generate the action potential whereas autonomic motoneurons seems to depend on the activity of Nav1.7 channels. | pt_BR |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES | pt_BR |
| dc.format | application/pdf | * |
| dc.language | por | pt_BR |
| dc.publisher | Universidade Federal do Triângulo Mineiro | pt_BR |
| dc.publisher.department | Pró-Reitoria de Pesquisa e Pós-Graduação | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.initials | UFTM | pt_BR |
| dc.publisher.program | Programa de Pós-Graduação em Ciências Fisiológicas | pt_BR |
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| dc.rights | Acesso Aberto | pt_BR |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
| dc.subject | NAO TEM | pt_BR |
| dc.subject.cnpq | NAO TEM | pt_BR |
| dc.title | Imunolocalização de canais para sódio voltagem-dependentes em neurônios dos núcleos ambíguo e dorsal motor do vago | pt_BR |
| dc.type | Dissertação | pt_BR |
| Aparece nas coleções: | Programa de Pós-Graduação em Ciências Fisiológicas | |
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| Dissert Lívia A Martins.pdf | Dissert Lívia A Martins | 2,55 MB | Adobe PDF |  Visualizar/Abrir |
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