Terapia fotodinâmica mediada por ZnPc Lipossomal sobre Candida albicans

Abstract

In the present work empty liposomes were stable constructed and phthalocyanine containing zinc for the realization of photodynamic therapy of Candida albicans. Experimental procedures included obtaining a standard suspension of C. albicans containing 106 viable cells per ml, the preparation of cells for testing and experiment conducted in five experimental groups: CG; GTFD; GLUZ; GDROGA and GLIPO in quadruplicate. The analyzes covered the determination of the number of colony forming units - (CFU); Cell growth curve, evaluation of pH of the culture medium and consumption of glucose during the growth of this fungus species. The construction of liposomes results showed a production of spherical vesicles with intact structures, unilamellar, homogeneous, with dimensions of 0.1 μm, to physical, chemical and biological stability to achieve the experimental procedures. To determine the number of colony forming units - CFU - the results showed a statistically significant difference between the experimental groups. ANOVA (p <0.05), Tukey's test. n =15. In this study, there was a 1.42 log reduction of CFU / ml in PDT group compared with group C. Through the growth curve of C. albicans planktonic in medium for 24 hours, a decrease of cell growth was demonstrated in GTFD compared to the group; C; DRUG; LIPO and LIGHT. A significant decline of pH occurred during the log phase of growth in the groups; C; DRUG; LIPO and LIGHT, with a pH acid measured from the 12 th h by the end of the 24th h. In GTFD the alkaline neutral pH remained unchanged (phase "lag") until the 13th hour. After a final pH of 5.51 acid at 24 h. We found a significant decrease in the concentration of glucose in groups C; DRUG; LIPO and LIGHT at all times where the sample was taken (24 h). From which no glucose consumption indications. GTFD observed in a reduced consumption of glucose during the period of 24 hours. The comparison between the groups for the growth curve variables, pH and glucose concentration measurements were performed by ANOVA, p <0.05, Tukey test. The GTFD showed statistically significant differences when compared to the group: C; DRUG; LIPO and LIGHT.