Avaliação do efeito do soro total e de frações obtidas do látex da Seringueira (Hevea brasiliensis) na cicatrização de feridas em modelo experimental

Abstract

The latex extracted from the rubber tree (Hevea brasiliensis) showed economic prominence in the 19th and 20th centuries due to its main product, rubber. Based on the findings regarding beneficial biological properties by latex of rubber tree, a number of important researches in the medical field were carried out in search of different clinical applications related, mainly wound healing. In this context, this work aimed to evaluate the effect of total serum and fractions obtained from rubber latex on wound healing in an experimental model. Initially the total serum was extracted from the latex by the coagulation of the latex, addition of 5% acetic acid, and by means of ion exchange chromatography, three protein fractions denominated FrHB1, FrHB2 and FrHB3 were obtained. Such fractions, as well as total serum, were tested on McCoy cell culture and the growth of these fibroblasts were evaluated. The cutaneous wound healing assay was performed in mice, where 5 groups of 8 animals each were treated with different concentrations of FrHB1 and a total serum concentration, in addition to a control group treated with the base gel alone. From fragments removed from the neoformed tissue in the animals, histological analysis, collagen quantification and measurement of inflammatory mediators were done. In relation to fibroblast cell growth, a tendency of FrHB1 0,01% to increase the amount of cells in culture was observed. The lowest concentration of FrHB1 tested (0.01% w/w) showed a more favorable activity in relation to the reduction of wound closure time, confirmed by the histological analysis. The collagen production was increased in all treated groups, especially in that treated with FrHB1 0,01% as shown by the histological analysis and corroborated by the calculation of the collagen content. The dosed cytokines did not present conclusive results. In this way, knowing the healing activity of the latex, described in this study, it is necessary to do more research that seeks to broaden the knowledge spectrum in relation to rubber latex, contributing to the elaboration of a herbal medicine based on Hevea brasiliensis.