Biblioteca Digital de Teses e Dissertações PÓS-GRADUAÇÃO SCTRICTO SENSU Programa de Mestrado Profissional em Inovações e Tecnologias
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dc.creatorVILAS BOAS, Douglas Fabiano-
dc.creator.ID06521228684por
dc.creator.Latteshttp://lattes.cnpq.br/7460932604899049por
dc.contributor.advisor1MALPASS, Ana Claudia Granato-
dc.contributor.advisor1ID24699866808por
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/0584086832551726por
dc.contributor.advisor-co1OKURA, Monica Hitomi-
dc.contributor.advisor-co1ID10346433800por
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/4554531240354966por
dc.contributor.advisor-co2SOUSA, Cristina Paiva de-
dc.contributor.advisor-co2ID17676533449por
dc.contributor.advisor-co2Latteshttp://lattes.cnpq.br/9002619114161319por
dc.date.accessioned2018-06-04T18:12:07Z-
dc.date.issued2017-08-04-
dc.identifier.citationVILAS BOAS, Douglas Fabiano. Isolamento biomonitorado de composto antibacteriano produzido por Streptomyces tubercidicus. 2017. 66f. Dissertação Mestrado em Inovação Tecnológica) - Programa de Mestrado Profissional em Inovação Tecnológica, Universidade Federal do Triângulo Mineiro, Uberaba, 2017.por
dc.identifier.urihttp://bdtd.uftm.edu.br/handle/tede/568-
dc.description.resumoOs microrganismos endofíticos apresentam uma grande diversidade genética e são importantes fontes para a descoberta de novos metabólitos secundários bioativos. Os produtos naturais de endofíticos apresentam amplo espectro de atividade biológica, incluindo antibióticos, imunossupressores, anticancerígenos, antioxidantes e outros. Neste trabalho reproduziu-se o cultivo do microrganismo endofítico Streptomyces tubercidicus isolado da planta Solanum lycocarpum St. Hill (Lobeira) do Cerrado da cidade de São Carlos - SP, que faz parte do campus da Universidade Federal de São Carlos – UFSCar, seguindo as condições previamente descritas. Os resultados do cultivo demonstraram quea linhagem é bastante resistente ao tempo de armazenamento, pois quase dez anos após seu isolamento e estudos realizados por Ratti et al., 2008. O processo de separação e purificação por técnicas cromatográficas do extrato bioativo de S. tubercidicus, levou ao isolamento de uma fração ativa, a qual apresenta atividade antibacteriana contra bactérias Gram + e Gram -. Esse resultado é diferente do obtido anteriormente, pois no estudo anterior foram obtidas duas frações ativas, porém uma com atividade contra a Escherichia coli, bactéria Gram + e outra com atividade contra bactéria Staphylococcus aureus, bactéria Gram -. O processo de elucidação estrutural do metabólito secundário bioativo isolado de S. tubercidicus ainda não foi finalizado, mas é possível concluir que não foi isolada a tubercidina composto bioativo produzido pelo S. tubercidicus, pois os dados dos espectros de RMN de 1H e de 13C do composto majoritário isolado são bastante distintos dos dados simulados para a tubercidina.por
dc.description.abstractEndophytic microorganisms present a great genetic diversity and are an important source for the discovery of new bioactive secondary metabolites. Natural endophytic products exhibit a broad spectrum of biological activity, including antibiotics, immunosuppressants, anticancer drugs, antioxidants and others.In this study, the endophytic microorganism Streptomyces tubercidicus, isolated from the Solanum lycocarpum St. Hill (Lobeira) plant of the Cerrado of the Universidade Federal de São Carlos (UFSCar),was cultivated and the results demonstrate that the lineage is quite resistant to storage time, as it was isolated almost ten years before the present cultivation. The process of separation and purification of the bioactive extract of S. tubercidicus led to the isolation of only one active fraction. This fraction displays antibacterial activity against Gram + and Gram - bacteria. This result is different from that previously obtained, where two active fractions were obtained, but one with activity against Gram + bacteria and another with activity against Gram - bacteria.The structural elucidation of the bioactive secondary metabolite isolated from S. tubercidicus has not yet been concluded, but it can be concluded that tubercidin has not been isolated as the 1H and 13C NMR spectra of the isolated major compound are distinct from simulated data for tubercidin.eng
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Geraispor
dc.formatapplication/pdf*
dc.thumbnail.urlhttp://bdtd.uftm.edu.br/retrieve/3482/Dissert%20Douglas%20F%20V%20Boas.pdf.jpg*
dc.languageporpor
dc.publisherUniversidade Federal do Triângulo Mineiropor
dc.publisher.departmentInstituto de Ciências Tecnológicas e Exatas - ICTE::Programa de Mestrado Profissional em Inovação Tecnológicapor
dc.publisher.countryBrasilpor
dc.publisher.initialsUFTMpor
dc.publisher.programPrograma de Mestrado Profissional em Inovação Tecnológicapor
dc.relation.referencesACS G, REICH E, MORI M. Biological and biochemical properties of the analogue antibiotic tubercidin. Proceedings of the National Academy of Sciences of the USA,v.52, p.493–501,1964. ALVES, A. R. F. Doenças alimentares de origem bacteriana. 87f. Dissertação (Mestrado em Ciências Farmacêuticas). Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Porto, 2012. ANZAI K, NAKAMURA G, SUZUKI S. A new antibiotic, tubercidin. Journal of Antibiotics,v.10, p.201–204, 1957. AOKI J. I.; YAMASHIRO-KANASHIRO E. H.; RAMOS, D. C. C.; COTRIM, P. C. Efficacy of the tubercidin antileishmania action associated with an inhibitor of the nucleoside transport. Parasitology Research,v.104, p.223–228,2009. BAE, H.; KIM S.; SICHER JR, R.C.; KIM M.S.; STREM, M.D.; BAILEY, B.A. The drought response of Theobroma cacao (cacao) and the regulation of genes involved in polyamine biosynthesis by drought and other stresses. Biological Control,v.46, p.24-28,2008. BALDANI, J.I.; BALDANI, V.L.D.; SELDIN, L.;DOBEREINER, J. Characterization of Herbaspirillurn seropedicae gen. nov. sp. nov. a Root- Associated Nitrogen-Fixing Bacterium. International Journal of Systematic Bacteriology,v.36, p.86–93,1986. BARRAQUIO, W.L.; REVILLA, L.; LADHA, J.K.Isolation of endophytic diazotrophic bacteria from wetland rice.Plant Soil, v.194, p.139–149,1997. BLACKWELL, M.Terrestrial Life—Fungal from the Start? Science, v.15, n.289, p.1884-1885, 2000. BODDEY, R.M.; URQUIAGA, S.; ALVES, B.J.R.; REIS, V. Endophytic nitrogen fixation in sugarcane: present knowledge and future applications. Plant Soil, v.252, p.139–149,2003. CASTILLO, U.F.; STROBEL, G.A.; MULLENBERG, K.; CONDRON, M.M.; TEPLOW, D.B.; FOLGIANO, V.; GALLO, M.; FERRACANE, R.; MANNINA, L.; VIEL, S.; CODDE, M.; ROBISON, R.; PORTER, H.; JENSEN, J. Munumbicins E-4 and E-5: novel broad-spectrum antibiotics from Streptomyces NRRL3052. FEMS Microbiology Letters,v.255, p.296–300,2006. CHALLIS, G. L.; HOPWOOD, D. A. Synergy and contingency as driving forces for the evolution of multiple secondary metabolites productions by Streptomyces species. PNAS, v.100, p.14555-14561, 2003. CLSI. Clinical and Laboratory Standards Institute. Antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Norma aprovada 3ª ed. Wayne, PA, CLSI document M31-A3, 2008. CRUVINEL, A,R; SILVEIRA, A,R, SOARES, J.S. perfil antimicrobiano de staphylococcus aureus isolado de pacientes hospitalizados em uti no distrito federal. Cenarium Pharmacêutico, v.4, n° 4, p.11-15, 2011. DAI, C.C.; YU, B.Y.; ZHAO, Y.T.; JIANG, J.H.; YANG, Q.Y. The screening and identification of endophytic fungi from four species of family Euphorbiaceae and the strain sp. antibacterial activity.Journal of Nanjing Forestry University,v.30, p.79– 83,2006. EZRA, D.; CASTILLO, U.F.; STROBEL, G.A.; HESS, W.M.; PORTER, H.; JENSEN, J.B.; CONDRON, M.A.; TEPLOW, B.; SEARS, J.; MARANTA, M.; HUNTER, M.; WEBER, B.; YAVER, D. Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp. Archives of Microbiology,v.150, p.785–793,2004. GASONI, L.; DE GURFMKEL, B.S. The endophyte Cladorrhinum foecundissimum in cotton roots: phosphorus uptake and host growth. Mycological Research, v.101, p.867–870,1997 GERHARDT, P.; MURRAY, G.G.E.; WOOD, W.A.; KRIEG, N.R. Methods for general and molecular microbiology. In ASM publication, Washington, D.C., 791 p.1994. GUO, B.; WANG, Y.; SUN, X.; TANG, K.Bioactive Natural Products from Endophytes: A review. Applied Biochemistry and Microbiology,v.44, n.2, p.136– 142,2008. ISHII, T.; NONAKA, K.; SUGA, T.; MASUMA, R.; OMURA, S.; SHIOMI, K. Cytosporone S with antimicrobial activity, isolated from the fungus Trichoderma sp. FKI-6626. Bioorganic & Medicinal Chemistry Letters, v.23, p.679–681,2013. KAPER JB, NATARO JP, MOBLEY HLT. Pathogenic Escherichia coli. Nat Rev Microbiol. 2004;2:123-40 KIM, S.; SHIN, D.S.; LEE, T.; OH, K.B. Periconicins, two new fusicoccane diterpenes produced by an endophytic fungus Periconia sp. with antibacterial activity. Journal of Natural Products,v.67, n.3, p.448-450,2004. LEE, J.; SEO, H.; YOON, S.; CHOI, K.; LEE, C.; RHEE, H. The synthesis and evaluation of new carbocyclic pyrrolo-[2,3-d]-pyrimidine nucleoside analogs Heterocycles, v.89, n.7, p.1557-1584,2014. LEITE, GUSTAVO BALDUINO. Análise de portadores assintomáticos de Staphylococcus aureus no Hospital Universitário de Brasília. Brasília, 2008. 101 f. LOIRET, F.G.; ORTEGA, E.; KLEINER, D.; ORTEGA-RODES, P.; RODES, R.;DONG, Z. A putative new endophytic nitrogen–fixing bacterium Pantoea sp. from sugarcane. Journal of Applied Microbiology,v.97, p.504–511,2004. LU, H.; ZOU, W.X.; MENG, J.C.; HU, J.; TAN, R.X. New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua. Plant Science,v.151, p.67–73,2000. MALINOWSKI, D.P.; BRAUER, D.K.; BELESKY, D.P.The Endophyte Neotyphodium coenophialum Affects Root Morphology of Tall Fescue Grown under Phosphorus Deficiency. Journal of Agronomy and Crop Science,v.183, p.53–60,1999. MANGUIAT LS, FANG TJ. Microbiological quality of chicken and pork-based streetvended foods from Taichung, Taiwan, and Laguna, Philippines. Food Microbiol. 2013;36(1):57-62. [DOI: 10.1016/j. fm.2013.04.005]. MCCARTY, R. M.; BANDARIAN, V. Biosynthesis of pirrolopyrimidines. Bioorganic Chemistry, v.43, p.15-25, 2012. MITCHELL, S. S.; POMERANTZ, S. C.; CONCEPCIÓN, G. P.; IRELAND, C. M.Tubercidin Analogs from the Ascidian Didemnum voeltzkowi.Journal of Natural Products,v.59, p.1000-1001,1996. MOOBERRY, S. L.; STRATMAN, K.; MOORE, R. E. Tubercidin stabilizes microtubules against vinblastine-induced depolymerization, a taxol-like effect. Cancer Letters,v.96,p.26 l-266,1995. NEWMAN, L.A.; REYNOLDS, C.M. Bacteria and phytoremediation: new uses for endophytic bacteria in plants.Trends in Biotechnology,v.23, p.6–8,2005. NISHIMURA H, KATAGIRI K, SATO K, MAYAMA M, SHIMAOKA N. Toyocamycin, a new anti-candida antibiotics. Journal of Antibiotics,v.9, p.60–62,1956. OSTROSKY, E.A.; MIZUMOTO, M.K.; LIMA, M.E.L.; KANECO, T.M.; NISHIKAWA, S.O.; FREITAS, B.R. Métodos para a avaliação da atividade antimicrobiana e determinação da concentração mínima inibitória (CIM) de plantas medicinais. Revista Brasileira de Farmacognosia, São Paulo, v.18, n.2, 2008. RAO, K.; RENN, D. BA-90912: An antitumor substance. Antimicrobial Agents and Chemotherapy,v.161, p.77–79,1963. RATTI, R. P.; SERRANO, N.F.G.; HOKKA, C.O.; SOUSA, C.P. Antagonistic properties of some microorganisms isolated from Brazilian tropical savannah plants against Staphylococcus coagulase-positive strain.Journal of Venomous Animals and Toxins including tropical Disease, v.14, n.2, p.294-302,2008. RATTI, R. P.; SOUSA, C. P. Staphylococcus aureus meticilina resistente (MRSA) e infecções nosocomiais. Revista de Ciências Farmacêuticas Básica e Aplicada. v.30, n.2, p.1-8, 2009. RATTI, R.; TOLEDO PIZA A.C.M.; GRANATO, A. C.; HOKKA, C.O.; DUBREUIL, J.D.; PAIVA DE SOUSA, C. Growing kinetics and antimicrobial activity of S. tubercidicus against pathogenic bacteria and fungi. Current Research Topics in Applied Microbiology and Microbial Biotechnology. Singapura: World Scientific Publishing Co., 2010, 589-592. RAYALA, R.; THEARD, P.; ORTIZ, H.; YAO, S.; YOUNG, J. D.; BALZARINI, J.; ROBINS, M. J.; WNUK, S. F. Synthesis of Purine and 7-Deazapurine Nucleoside Analogues of 6-N-(4-Nitrobenzyl)adenosine; Inhibition of Nucleoside Transport and Proliferation of Cancer Cells. Chem Med Chem, v.9, p.2186–2192,2014. ROSADO, A.S. E SELDIN, L. Production of a potencially novel anti-microbial substance by Bacillus polymyxa. World Journal of Microbiology and Biotechnology, v.90, p.521-528, 1993. ROSSI, F.S.; Ceccon, M.E.J.R.; Krebs, V.L.. Infecções Estafilocócicas Adquiridas nas Unidades de Terapia Intensiva Neonatais. Pediatria, São Paulo, v. 27, n. 1, p. 38-47, 2005. RUBINI, M.R.; SILVA-RIBEIRO, R.T.; POMELLA, A.W.V.; MAKI, C.S.; ARAUJO, W.L.; DOS SANTOS; D.R.;AZEVEDO, J.L. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of Witches' Broom Disease.International Journal of Biological Science,v.1, p.24–33,2005. RYAN R.P.; GERMAINE, K.; FRANKS A.; RYAN D.J.; DOWLING D.N. Bacterial endophytes: recent developments and applications. FEMS Microbiology Letters,v.278, p.1–9,2008. SANDHIYA, G.S.; SUGITHA, T.C.; BALACHANDAR, D.;KUMAR, K. Endophytic colonization and in planta nitrogen fixation by a diazotrophic Serratia sp. in rice. Indian Journal of Experimental Biology,v.43, p.802- 807,2005. SANEYOSHI M, TOKUZEN R, FUKORA F. Antitumor Activities and Structural Relationship of Tubercidine Toyocamycin and Their Derivatives. Gann,v.56, p.219- 223, 1965. SHIRLING, E.B.; GOTTLIEB, D. Methods for Characterization of Streptomyces Species.International Journal of Systematic Bacteriology,v.16,p.313-340,1966. SILVERSTEIN, R. M.; BASSLER, G. C.; MORRILL, T. C. Spectrometric Identification of Organic Compounds, 5a. edição, John Wiley & Sons, Inc. EUA, 1991. SMITH, C. G.; LEE, S. J.; MARQUARDT, D. L.Effects of Tubercidin and its 5’-OMethyl Ether on Adenosine Receptors and Mediator Release Functions in Mast Cells. Journal of Medicinal Chemistry,v.38,p.2259-2262,1995. STROBEL, G.; FORD, E.; WORAPONG, J.; HARPER, J.K., ARIF, A.M.; GRANT, D.M.; FUNG, P.C.W.; CHAU, R.M.W.Isopestacin, an isobenzofuranone from Pestalotiopsis microspora, possessing antifungal and antioxidant activities. Phytochemistry,v.60, p.179,2002. STROBEL, G; DAISY B.; CASTILLO U.; HARPER J. Natural products from endophytic microorganisms. Journal of Natural Products,v.67, p.257-268 ,2004. SUMARAH, M.W.; MILLER, J.D.; ADAMS, G.W. Measurement of a rugulosinproducing endophyte in white spruce seedlings.Mycologia,v.97, p.770–776,2005. TEJESVI, M.V.; NALINI, M.S.; MAHESH, B.; PRAKASH, H.S.; KINI, K.R.; SHETTY, H.S.; SUBBIAH, V. New hopes from endophytic fungal secondary metabolites. Boletín de laSociedad Química de México,v.1, p.19-26, 2007. TORTORA GJ, FUNKE BR, CASE CL. Microbiologia. 6. ed. Porto Alegre, ArTmed; 2000. VAN AKEN, B. Biodegradation of nitro-substituted explosives 2,4,6-trinitrotoluene, hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5- tetrazocine by a phytosymbiotic Methylobacterium sp. associated with poplar tissues (Populus deltoides × nigra DN34). Applied and Environmental Microbiology,v.70, p.508–517,2004. WANG, X.P.; ZHONG, J.; WANG, X.H.; LI, M.X.; ZHANG, L.Q.Study on the bioactivity of the endophytic fungi isolated from Sinopodophyllum hexandrum and Diphylleia sinensis. Natural Product Research and Development,v.8, p.15–19,2006. WEBER, D.; STERNER, O.; ANKE, T.; GORZALCZANCY, S.; MARTIN, V.; ACEVEDO, C. Phomol, a new antinflammatory metabolite from an endophyte of the medicinal plant Erythrina crista-galli.Journal of Antibiotics,57, 559–563,2004. WICKLOW, D.T.; ROTH, S.; DEYRUP, S.T.; GLOER, J.B. A protective endophyte of maize: Acremonium zeae antibiotics inhibitory to Aspergillus flavus and Fusarium verticillioides. Mycological Research,v.109, p.610–618,2005. WILLIAMS MD, WILKINS ST. Bergey's. Manual of Determinative Bacteriology. 9th ed., Baltimore; 1994. WOLBERG WH. Effect of tubercidin on nucleoside incorporation in human tumors. Biochemical. Pharmacology,v.14, p.1921–1925,1965. YANG, H.; BUDOW-BUSSE, S.; EICKMEIER, H.; REUTERC, H.; SEELA, F. 7- Cyclopropyl-2-deoxytubercidin: a carbocyclic side-chain derivative of 7-deaza-200-deoxyadenosine. Acta Crystallographica, p.1-5, 2014. ZABEL, V.; SAENGER, W.; SEELA, F. Structures of 2'-deoxytubercidin (I) and 2'- deoxytubercidin dihydrate (II).Acta Crystallographic, p.131–134, 1987. ZENG, S.R.; XU, Q.W.; YE, B.T.; KE, Y.; FANG, B.Y.; HUANG, X.M. Isolation of endophytic fungi from Polygonum cuspidatum sieb.et zucc.and screening of isolates62 producing antibiotic active substances. Journal of Fungal Research,v.3, p.24–26, 2005. ZHANG, H.W.; SONG, Y.C.; TAN, R.X. Biology and chemistry of endophytes.Natural Products Report,v.23, p.753–771,2006. ZHANG, L.; ZHANG, Y.; LI, X.; ZHANG, L. Study on the Synthesis and PKA-I Binding Activities of 5-Alkynyl Tubercidin Analogues. Bioorganic & Medicinal Chemistry,v.10, p. 907–912, 2002.por
dc.rightsAcesso Abertopor
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.subjectMicrorganismos endofíticos.por
dc.subjectAtividade antibacteriana.por
dc.subjectRMN.por
dc.subjectEndophytic microorganisms.eng
dc.subjectAntibacterial activity.eng
dc.subjectNMR.eng
dc.subject.cnpqBioquímicapor
dc.titleIsolamento biomonitorado de composto antibacteriano produzido por Streptomyces tubercidicuspor
dc.typeDissertaçãopor
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