Avaliação dos efeitos imunológicos da terapia fotodinâmica mediada por ALA em camundongos infectados com Leishmania braziliensis

Abstract

Introduction: Photodynamic therapy (PDT) has been shown because it offers an efficient alternative for the leishmaniasis. The cutaneous injuries create high edged ulcers, well defined and with granular aspect. IL-22 is associated to skin and mucous membrane and connect to the patients protective profile with calazar by L. (L.) donovani. In this study, it was evaluated the parasite load and the possible modulation of immune response after TFD use through aminolevulinic acid in Leishmania braziliensis infected mice. Methods: Were used for this study 48 mice infected with L. braziliensis divided into four groups with different types of treatment, untreated group, group treated with ALAtreated group light group treated with PDT (ALA + LIGHT). After treatment the animals were euthanized, and their paws removed for counting a count of the parasites by light microscopy, mast analysis of histological sections stained with Giemsa and analysis of mRNA expression of IFN- -4, IL-17, IL- 22, IL-27, IL-10 and transcription factors GATA-3 and T-bet Foxp3 by RT-PCR. Results: The treated group parasite load was significantly smaller compared to the non-treated one (p<0.0001) and the group treated only with light or ALA (p= 0.0001). No significant differences in the quantification of mast cells. There were significant decreased in the IL-22 expression in animals treated with light (p= 0.0061) and with PDT (p= 0.0049). Conclusion: Photodynamic therapy was efficient connected when comes to decreased of parasite load and there was a decrease in the IL-22 expression in the PDT treated group.