Correlação de citocinas e óxido nítrico teciduais com fatores prognósticos em pacientes com neoplasias malignas de ovário

Abstract

The objective of this study was to correlate tissue marking by immunohistochemistry of cytokines (IL2, IL5, IL6, IL8, IL10, TNF-α) and iNOS with clinical and pathological parameters of patients with primary ovarian malignancy. As material and methods, were prospectively evaluated 40 patients undergoing surgical treatment for ovarian cysts in accordance with pre-established criteria and with confirmed diagnosis of ovarian cancer. Immunohistochemistry study for cytokines (IL2, IL5, IL6, IL8, IL10, TNF-α) and iNOS was performed. The evaluation of prognostic factors was performed using the Fisher’s exact test. The significance level was less than 0.05. As result it was found that the histological grade 1 was significantly correlated with immunohistochemical of intensity (2 and 3) for TNF-α (p=0.0028). Also in relation to TNF-α, early stages (I and II, FIGO) were more related to the strong intensity (2 and 3) of immunohistochemical labeling limit breadth of significance (p=0.0525). Strong staining immunohistochemical IL5 (2 and 3) was related to disease-free survival less than or equal to 24 months, suggesting that a factor of poor prognosis, but there was no statistical significance (p = 0.1771). There was no statistical significance in relation at other cytokines studied and iNOS. The study concluded that the immunohistochemical staining in strong intensity for TNF-α is related to ovarian cancer in histological grade 1 and stages I and II (FIGO). The study of immunology in ovarian cancer may help to better understand the patterns of tumor response to various treatments. Therefore, the study of new prognostic markers in primary cancer of the ovary is important for better planning of surgical and adjuvant treatment of patients since the treatment of this malignancy is still a challenge in Gynecologic Oncology.