Papel funcional das rocks sobre a proliferação de células de carcinoma epidermoide oral in vitro

Abstract

The oral carcinoma cell squamous (OSCC) is epithelial cancer responsible for the majority of malignant lesions affecting the head and neck region. The HA-1077 and the Y-27632 are inhibitors of the ROCKs which are effector of Rho GTPases, and are associated with pathogenesis and progression of human tumors. This study aimed to evaluate the functional role of the ROCKs in the regulation of cellular proliferation of SCC-4 cell line from OSCC. Control cells and cells treated with HA-1077 (25, 50 and 100 μM) were quantified for 6 and 24 hours; and HA-1077 (25, 50 and 100 μM), HA-1077 50 µM and/or Y-27632 30 µM for 6 hours, in BrdU assays and immunoexpression of KI-67 assays. Was considered significant p<0.05. The number of SCC-4 cells treated with HA-1077 (25, 50 and 100 μM) that incorporated BrdU was significantly lower than control cells in 6 hours [F (3.11) = 107.461, p<0.0001] and 24 hours [F (3.11) = 167.433, p<0.0001], therefore dose dependent. The number of proliferative SCC-4 cells treated with HA-1077 (25, 50 and 100 μM), HA-1077 50 µM and/or Y-27632, for 6 hours, in BrdU assay [F (5.17) = 443.818, p<0.0001], and in imunoexpressaram KI-67 assay, also, was significantly less [F (5.17) = 192.595, p<0.0001], than control cells. The results obtained suggest that the ROCKs play an important functional role in the regulation of cellular proliferation in OSCC, since both HA-1077 and/or the Y- 27632, inhibitors of ROCKs, have an inhibitory effect on proliferation of SCC-4 cells.