Use este identificador para citar ou linkar para este item:
http://bdtd.uftm.edu.br/handle/tede/832Registro completo de metadados
| Campo DC | Valor | Idioma |
|---|---|---|
| dc.creator | ARAÚJO, Márcia Fernandes de | - |
| dc.creator.ID | 26262621881 | por |
| dc.creator.Lattes | http://lattes.cnpq.br/3777111464869341 | por |
| dc.contributor.advisor1 | PEREIRA, Sanívia Aparecida de Lima | - |
| dc.contributor.advisor1ID | 66141583687 | por |
| dc.contributor.advisor1Lattes | http://lattes.cnpq.br/9567264526096951 | por |
| dc.date.accessioned | 2019-08-26T14:35:10Z | - |
| dc.date.issued | 2013-02-01 | - |
| dc.identifier.citation | ARAÚJO, Márcia Fernandes de. Avaliação da mucosa periimplantar em pacientes com periimplantite: aspectos clínicos, histopatológicos e imunológicos. 2013. 70f . Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-graduação em Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, 2013 . | por |
| dc.identifier.uri | http://bdtd.uftm.edu.br/handle/tede/832 | - |
| dc.description.resumo | A periimplantite é uma lesão que circunda os implantes dentários devido a fatores como esforços mecânicos excessivos, corrosão e presença de bactérias agressivas, podendo provocar infecção e inflamação, além de perda óssea em torno dos implantes osseointegrados. Objetivo – O objetivo deste trabalho foi comparar a resposta inflamatória na mucosa periimplantar entre pacientes com periimplantite (CP) e pacientes sem periimplantite (SP). Material e Métodos – Foi realizada biópsia da mucosa periimplantar de 18 pacientes: nove do grupo CP e nove do grupo SP. Em cada caso, foram coletados e processados dois fragmentos. Foram realizados cortes seriados para análise histopatológica e imuno-histoquímica. Um dos fragmentos foi destinado para análise histopatológica, utilizando as colorações de hematoxilina-eosina e picro-sírius para quantificar a densidade de células inflamatórias e fibras colágenas, respectivamente. A análise imuno-histoquímica foi realizada para TGF-β , IL-17, CD31, mastócito-quimase e mastócito-triptase. O outro fragmento foi utilizado para quantificar a expressão de IL-13 por imunoensaio enzimático (ELISA). Resultados – A densidade de células imunomarcadas para TGF-β e IL-17 e a densidade de vasos imunomarcados para CD31 foi significativamente maior no grupo CP quando comparado ao grupo SP. O grupo CP apresentou maior densidade de leucócitos, mastócito-quimase e mástócito-triptase, embora sem diferença significativa. Os pacientes do grupo SP apresentaram maior expressão de IL-13 e maior quantidade de fibras colágenas quando comparados aos pacientes do grupo CP, com diferença significativa. Nenhum dos pacientes apresentou supuração ou necrose. No grupo CP, houve correlação positiva e significativa entre a densidade de mastócito-triptase e a densidade de vasos imunomarcados por anti-CD31. Houve também no grupo CP correlação positiva e significativa entre a densidade de mastócito-quimase e a densidade de vasos imunomarcados por anti-CD31. Houve correlação negativa e significativa entre a densidade de IL-17 e a porcentagem de colágeno também no grupo CP. Conclusões – A maior resposta inflamatória no grupo CP sugere a participação de mastócitos-triptase e mastócito-quimase na indução de alterações nas células endoteliais e maior expressão de CD31. Essa indução faz com que ocorra maior exsudação de leucócitos, o que aumentaria outras citocinas como a IL-17, que por sua vez agravaria o processo inflamatório e inibiria a atuação de IL-13. Com a redução de IL-13 no grupo CP, a ação pró-fibrótica neste grupo também estaria reduzida. Além disso, o aumento da IL-17 poderia ativar osteoclastos com a consequente perda de osso, o que poderia provocar a perda de implantes dentários. Dessa forma, com o estudo da influência de citocinas na periimplantite, novas terapias poderiam ser desenvolvidas, contribuindo assim com o aumento da longevidade do implante. | por |
| dc.description.abstract | The periimplantitis is a lesion surrounding dental implants due to factors such as excessive mechanical stress, corrosion and aggressive bacteria, which can cause infection and inflammation, and bone loss around dental implants. Objective – The aim of this study was to compare the inflammatory response in peri-implant gingival tissue between the group of patients with peri-implantitis (PP) and group with healthy healthy peri-implant tissues (HP). Material e Methods – Biopsy was performed of peri-implant mucosa of 18 patients: nine of PP group and nine of HP group. In each case two fragments were collected and serial sections were performed for histological and immunohistochemical analysis. One of the fragments was collected for the histopathological analysis, with use of hematoxylin-eosin (HE) and picro-sírius to quantify the density of inflammatory cells and collagen fibers respectively. Immunohistochemical analysis was performed to TGF-β, IL-17, CD31, mast cell chymase, and mast cell tryptase. The other fragment was used to quantify the expression of IL-13 by Enzymatic Immunoabsorbent Assay (ELISA). Results – Immunostained cell density for TGF-β, IL-17 and CD31 was significantly higher in PP group when compared with HP group. PP group showed greater density of red cells, leukocytes, mast cells chymase and mast cell tryptase, however, without significant difference.HP group patients showed higher IL-13 expression and increased amount of collagen fibers when compared with PP group patients, with significant difference. None of the patients presented suppuration or necrosis. There was significant positive correlation between the mast cell tryptase density and density of blood vessels immunostained with anti-CD31 in the PP group. There was significant positive correlation between the mast cell chymase density and density of blood vessels immunostained with anti-CD31 in the PP group. There was significant negative correlation between the density of IL-17 and collagen. Conclusions - This study demonstrated that in patients with peri-implantitis there was an increase in TGF-β and IL-17, indicating that these cytokines are directly involved in the inflammatory process. In addition, it demonstrated that there was an increase in IL-13 in patients with health peri-implant tissues, which could explain the larger number of collagen fibers in this group, suggesting that this cytokine has anti-nflammatory action. Thus, understanding the influence of cytokines in the peri-implantitis installation, new therapies could be developed in order to inhibit the synthesis of IL-17 and induce synthesis of IL-13 in peri-implant tissue, contributing to increase the longevity of the implant. | eng |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | por |
| dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de Minas Gerais | por |
| dc.description.sponsorship | Universidade Federal do Triângulo Mineiro | por |
| dc.description.sponsorship | Universidade de Uberaba | por |
| dc.description.sponsorship | Fundação de Ensino e Pesquisa de Uberaba | por |
| dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico | por |
| dc.format | application/pdf | * |
| dc.thumbnail.url | http://bdtd.uftm.edu.br/retrieve/5530/Dissert%20Marcia%20F%20Araujo.pdf.jpg | * |
| dc.language | por | por |
| dc.publisher | Universidade Federal do Triângulo Mineiro | por |
| dc.publisher.department | Instituto de Ciências da Saúde - ICS::Programa de Pós-Graduação em Ciências da Saúde | por |
| dc.publisher.country | Brasil | por |
| dc.publisher.initials | UFTM | por |
| dc.publisher.program | Programa de Pós-Graduação em Ciências da Saúde | por |
| dc.relation.references | ABBAS, A. K.; LICHTMAN, A. H.; PILLAI, S. Imunologia celular e molecular. 6. ed. Rio de Janeiro: Elsevier Editora Ltda., 2008. 564p. ALBOUY, J.P. et al. Spontaneous progression of experimental peri-implantitis at implants with different surface characteristics: an experimental study in dogs. J Clin Periodontol, v.39, n.2, p.182-187, 2012. ALPAGOT, T. et al. The association between gingival crevicular fluid TGF-beta 1 levels and periodontal status in HIV-1(+) patients. Journal of Periodontology, v.79, n.1, p.123-30, 2008. AMIN, K. The role of mast cells in allergic inflammation. Respiratory Medicine, v.106, n.1, p.9-14, 2012. ATSUTA, I. et al. Ultrastructural localization of laminin-5 (gamma2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy. Biomaterials, v.26, n.32, p.6280–6287, 2005. BATISTA, A.C.; RODINI, C.O.; LARA, V.S. Quantification of mast cells in different stages of human periodontal disease. Oral Diseases, v.11, n.4, p.249-254, 2005. BERGLUNDH, T.; PERSSON, L.; KLINGE, B. A systematic review of the incidence of biological and technical complications in implant dentistry reported in prospective longitudinal studies of at least 5 years. Journal of Clinical Periodontology, v.29, n.3, p.197-212, 2002. BUDUNELI, N. et al. Evaluation of transforming growth factor-beta 1 level in crevicular fluid of cyclosporin A-treated patients. Journal of Periodontology, v.72, n.4, p.526531, 2001. BULLON, P. et al. Immunohistochemical analysis of soft tissues in implants with healthy and peri-implantitis condition, and aggressive periodontitis. Clinical Oral Implants Research, v.15, n.5, p.553-559, 2004. CARRANZA, F. A. Periodontia Clínica. 8. ed. Rio de Janeiro: Guanabara Koogan, 1997. 832p. CHAPLIN, D. D. Overview of the human immune response. The Journal of Allergy and Clinical Immunology, v.117, n.2, p.430-435, 2006. CHARALAMPAKIS, G. et al. Clinical and microbiological characteristics of peri-implantitis cases: a retrospective multicenter study. Clinical Oral Implants Research, v.23, n.9, p.1045-1054, 2012. CHATTERJEE, S. et al. Quantitative role of mast cells in odontogenic cystic enlargement. Brazilian Journal of Oral Sciences, v.7, n.27, p.1162-1665, 2008. CONSOLARO, A. et al. Saucerization of osseointegrated implants and planning of simultaneous orthodontic clinical cases. Dental Press Journal of Orthodontics, v.15, n.3, p.19-30, 2010. COSYN, J. et al. The Peri-IPeriimplant Sulcus Compared with Internal Implant and Suprastructure Components: A Microbiological Analysis. Clinical Implant Dentistry and Related Research, v.13, n.4, p.286-295, 2011. CRISPIN, J.C.; TSOKOS, G.C. Interleukin-17-producing T cells in lupus. Curr Opin Rheumatol, v.22, n.5, p.499-503, 2010. CZYZEWSKA-BUCZYŃSKA, A; WITKIEWICZ, W. Role of mast cells in the pathogenesis of atherosclerosis. Przegl Lek. v.68, n.3, p.171-174, 2011. DUARTE, P. M. et al. Effect of anti-infective mechanical therapy on clinical parameters and cytokine levels in human peri-iperiimplant diseases. Journal of Periodontology, v.80, n.2, p.234-243, 2009. FAVA, R. A. et al. Transforming growth factor beta 1 (TGF-beta 1) induced neutrophil recruitment to synovial tissues: implications for TGF-beta-driven synovial inflammation and hyperplasia. The Journal of Experimental Medicine, v.173, n.5, p.1121-1132, 1991. FRANSSON, C. et al. Severity and pattern of periimplantitis – associated bone loss. Journal of Clinical Periodontology, v.37, n.5, p.442-448, 2010. FUSCHIOTTI, P. Role of IL-13 in systemic sclerosis. Cytokine, v.56, n.3, p.544-549, 2011. GARBUZENKO, E. et al Human mast cells stimulate fibroblast proliferation, collagen synthesis and lattice contraction: a direct role for mast cells in skin fibrosis. Clinical and Experimental Allergy, v.32, n.2, p.237-246, 2002. GARTNER, L. P.; HIATT, L. J. Tratado de histologia em cores. Rio de Janeiro: Guanabara Koogan, 2003. 456p. GONZALES, J. R. et al. Production of interleukin-13 is influenced by the interleukin-4 -34TT and -590TT genotype in patients with aggressive periodontitis. Scandinavian Journal of Immunology, v.73, n.2, p.128-134, 2011. GUALINI, F; BERGLUNDH, T. Immunohistochemical characteristics of inflammatory lesions at implants. Journal of Clinical Periodontology, v.30, n.1, p.14-18, 2003. GUTCHER, I., BECHER, B. APC-derived cytokines and T cell polarization in autoimmune inflammation. The Journal of Clinical Investigation, v.117, n.5, p.1119-1127, 2007. HAUSER-GERSPACH, I. et al. Influence of gaseous ozone in periimplantitis: bactericidal efficacy and cellular response. An in vitro study using titanium and zirconia. Clinical Oral Investigation, v.16, n.4, p.1049-1059, 2011. HEASMAN, P., ESMAIL, Z., BARCLAY, C. Peri-implant diseases. Dental Update, v.37, n.8, p.511-512, 2010. HEITZ-MAYFIELD, L. J. Peri-implant diseases: diagnosis and risk indicators. Journal of Clinical Periodontology, v.35, n.8, p.292-304, 2008. HOLZHAUSEN, M., et al. Nafamostat mesilate, a potent tryptase inhibitor, modulates periodontitis in rats. Clinical Oral Investigation, v.15, n.6, p.967-973, 2011. JUNG. S.R. et al. Histological analysis of soft and hard tissues in a periimplantitis Lesion: A Human Case Report. Implant Dentistry, v.21, n.3, p.186-189, 2012. KELLY, M. N. et al. A. Interleukin-17/interleukin-17 receptor-mediated signaling is important for generation of an optimal polymorphonuclear response against Toxoplasma gondii infection. Infection and Immunity, v.73, n.1, p.617-621, 2005. KOTAKE, S. et al. IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. The Journal of Clinical Investigation, v.103, n.9, p.1345-1352, 1999. KRISHNASWAMY, G. et al. The human mast cell: an overview. Methods Mol Biol, v.315, p.13-34, 2006. KUMAR, M. et al. Let-7 microRNA-mediated regulation of IL-13 and allergic airway inflammation. The Journal of Allergy and Clinical Immunology, v.128, n.5, p.1077-1085, 2011. LIN, S. K. et al. Immunolocalization of macrophages and transforming growth factor-beta 1 in induced rat periapical lesions. Journal of Endodontics, v.26, n.6, p.335-340, 2000. LINDHE, J.; KARRING, T.; LANG, N.P. Tratado de Periodontia Clínica e Implantodontia Oral. Rio de Janeiro: Guanabara Koogan, 2010. 1304p. LINDSTEDT, K.A.; MÄYRÄNPÄÄ, M.I.; KOVANEN, P.T. Mast Cells in vulnerable atherosclerotic plaques- a view to a kill. Journal of Cellular and Molecular Medicine, v.11, n.4, p.739-758, 2007. LISKMANN, S. et al. Correlation between clinical parameters and interleukin-6 and interleukin-10 levels in saliva from totally edentulous patients with peri-implant disease. The International Journal of Oral & Maxillofacial Implants, v.21, n.4, p.543-550, 2006. MALUF, L.C.; BARROS, J.A.; MACHADO FILHO, C.D.S. Mastocitose. Anais Brasileiros de Dermatologia, v.84, n.3, p.213-225, 2009. MÁRTON, I.J. et al. Distribution of interleukin-2 receptor alpha-chain and cells expressing major histocompatibility complex class II antigen in chronic human periapical lesions. Oral Microbiol Immunol, v.13, n.4, p.259-262, 1998. MAŚLIŃSKA, D. et al. Association of mast cells with calcification in the human pineal gland. Folia Neuropathologica, v.48, n.4, p. 276-282, 2010. MEDZHITOV, R. Recognition of microorganisms and activation of the immune response. Nature, v.449, n.7164, p.819-826, 2007. MENDONÇA, A.C. et al. Tumor necrosis factor-alpha levels after surgical anti-infective mechanical therapy for peri-implantitis: a 12-month follow-up. Journal of Periodontology, v.80, n.4, p.693-699, 2009. MIOSSEC, P. Interleukin-17 in rheumatoid arthritis: if T cells were to contribute to inflammation and destruction through synergy. Arthritis and Rheumatism, v. 48, n.3, p.594- 601, 2003. MISCH, C. E., et al. Implant success, survival, and failure: the International Congress of Oral Implantologists (ICOI) Pisa Consensus Conference. Implant Dentistry, v.17, n.1, p.5-15, 2008. MORANDINI, A.C. et al. Periodontal ligament and gingival fibroblasts participate in the production of TGF-β, interleukin (IL)-8 and IL-10. Brazilian Oral Research, v.25, n.2, p.157-62, 2011. MOSMANN, T. R.; COFFMAN, R. L. TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Annual Review of Immunology, v.7, p.145-173, 1989. MURPHY, K. et al. Imunobiologia de Janeway. Porto Alegre: Artmed, 2010. 885p. NETTO, J.N.S. et al. Evaluation of mast cells in periapical cysts, dentirerous cysts, and keratocystic odontogenic tumors. Journal of Oral Pathology & Medicine, v.41, p.630-636, 2012. OLIVEIRA, C.R. et al. Comparative immunohistochemical study of the presence of mast cells in apical granulomas and periapical cysts: possible role of mast cells in the course of human periapical lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod., v.97, n.1, p.59-63, 2004. OPPERMANN, R. V.; GOMES, S. C.; FIORINI, T. Epidemiologia e fatores de risco para as doenças periimplantares. R. Periodontia, v.18, n.4, p.14-21, 2008. O'REILLY, S; HÜGLE, T; VAN LAAR, JM. T cells in systemic sclerosis: a reappraisal. Rheumatology (Oxford), v.51, n.9, p.1540-1549, 2012 PAGE, R. The role of inflammatory mediators in the pathogenesis of periodontal disease. Journal of Periodontal Research, v.26, p.230-242, 1991. PARK, J. B.; LEE, Y.C. Replacing a failed implant adjacent to the implant-supported restoration in the anterior region after ridge augmentation procedure. Gerodontology, v.28, n.3, p.238-242, 2011. PEREIRA, S.A. et al. Morphometric study of the spleen in chronic Chagas' disease. American Journal of Tropical Medicine and Higiene, v.66, n.4, p.401-403, 2002. PETKOVIC-CURCIN, A. et al. Cytokines in pathogenesis of peri-implatitis. Vojnosanit Pregl Journal, v.39, n.5, p.435-440, 2011. PINHEIRO, S.W. et al. Morphometric study of the fibrosis and mast cell count the circular colon musculature of chronic Chagas pacients with and without megacolon. Revista da Sociedade Brasileira Medicina Tropica,v.36, n.4, 2003. REAL-OSUNA, J.; ALMENDROS-MARQUÉS, N.; GAY-ESCODA, C. Prevalence of complications after the oral rehabilitation with implant-supported hybrid prostheses. Medicina Oral, Patologia Oral y Cirurgía Bucal, v.17, n.1, p.116-121, 2012. REZENDE, C. P. et al. Peri-implantite. Revista Gaúcha de Odontologia. v.53, n.4, 2005. RODINI, C. O.; BATISTA, A.C.; LARA, V.S. Comparative immunohistochemical study of the presence of mast cells in apical granulomas and periapical cysts: possible role of mast cells in the course of human periapical lesions. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, v.97, n.1, p.59-63, 2004. SAINTRAIN, M. V.; DE SOUZA, E. H. Impact of tooth loss on the quality of life. Gerodontology, v.29, n.2, p.632-636, 2012. SALLUM, A. W.; SILVA FILHO, W. L. S.; SALLUM, E. A. Etiopatogenia da doença periimplantar. R. Periodontia, v.18, n.4, 2008. SÁNCHEZ-GARCÉS, M. A.; GAY-ESCODA, C. Periimplantitis. Medicina Oral, Patologia Oral y Cirurgía Bucal, v.9, p.69-74, 2004. SATO, K. et al. Th17 functions as an osteoclastogenic helper T cell subset that links T cell activation and bone destruction. The Journal of Experimental Medicine, v.203, n.12, p.2673-2682, 2006. SCOTTON, C. J.; CHAMBERS, R. C. Molecular targets in pulmonary fibrosis: the myofibroblast in focus. Chest. v.132, n.4, p.1311-1321, 2007. SÉGUIER, S. et al. Collagen fibers and inflammatory cells in healthy and diseased human gingival tissues: a comparative and quantitative study by immunohistochemistry and automated image analysis. J Periodontol, v.71, n.7, p.1079-1085, 2000. SEVERINO, V. O.; NAPIMOGA, M. H.; DE LIMA PEREIRA, S. A. Expression of IL-6, IL-10, IL-17 and IL-8 in the peri-implant crevicular fluido of patients with peri-implantitis. Archives of Oral Biology, v.56, n.8, p. 823-828, 2011. SHIBLI, J. A. et al. Composition of supra- and subgingival biofilm of subjects with healthy and diseased implants. Clinical Oral Implants Research, v.19, n.10, p.975-82, 2008. SHIH, D. Q., TARGAN, S. R., MCGOVERN, D. Recent advances in IBD pathogenesis: genetics and immunobiology. Current Gastroenterology Reports, v.10, n.6, p.568–575, 2008. SKALERIC, U. et al. Changes in TGF-beta 1 levels in gingiva, crevicular fluid and serum associated with periodontal inflammation in humans and dogs. European Journal of Oral Sciences, v.105, n.2, p.136-142, 1997. STOCKINGER, B.; VELDHOEN, M. Differentiation and function of Th17 T cells. Current Opinion in Immunology, v.19, n.3, p.281-286, 2007. SUAREZ, L. J. et al. Relative proportions of T-cell subpopulations and cytokines that mediate and regulate the adaptive immune response in patients with aggressive periodontitis. Journal of Periodontology, v.75, n.9, p.1209-1215, 2004. TETÈ, S. et al. Collagen fiber orientation around machined titanium and zirconia dental implant necks: an animal study. The International Journal of Oral & Maxillofacial Implants, v.24, n.1, p.52-58, 2009. WATANABE. et al. High density of tryptase-positive mast cells in patients with renal cell carcinoma on hemodialysis: correlation with expression of stem cell factor and protease activated receptor-2. Human Pathology,v.43, n.6, p.888-897, 2012. YAMANAKA, Y. et al. Expression of angiogenic factors in rat periapical lesions. Basic Research – Biology, v.38, n.3, p.313-317, 2012. YEUNG, S. C. Biological basis for soft tissue management in implant dentistry. Australian Dental Journal, v.53, p.39-42, 2008. YOUNES, R. et al. Pertinent cell population to characterize periodontal disease. Tissue Cell, v.41, n. 2, p. 141-150, 2009 ZITZMANN, N. U.; BERGLUNDH, T. Definition and prevalence of peri-implant disease. Journal of Clinical Periodontology, v.35, n.8, p.286-291, 2008. ZURAWKI, G.; VRIES, J. E. Interleukin13, an interleukin 4-like cytokine that acts on monocites and B cells, but not on T cells. Immunology Today, v.15, n.1, p.19-26, 1994. | por |
| dc.rights | Acesso Aberto | por |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
| dc.subject | Implantes dentários. | por |
| dc.subject | Periimplantitite. | por |
| dc.subject | Inflamação. | por |
| dc.subject | Citocinas. | por |
| dc.subject | Dental implants. | eng |
| dc.subject | Periimplantititis. | eng |
| dc.subject | Inflammation. | eng |
| dc.subject | Cytokines. | eng |
| dc.subject.cnpq | Anatomia Patológica e Patologia Clínica | por |
| dc.title | Avaliação da mucosa periimplantar em pacientes com periimplantite: aspectos clínicos, histopatológicos e imunológicos | por |
| dc.type | Dissertação | por |
| Aparece nas coleções: | Programa de Pós-Graduação em Ciências da Saúde | |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Dissert Marcia F Araujo.pdf | Dissert Marcia F Araujo | 1,15 MB | Adobe PDF |  Visualizar/Abrir |
Este item está licenciada sob uma Licença Creative Commons
