Expressão do hormônio liberador da corticotrofina (CRH) e seus receptores, e da proteína facilitadora do transporte da glicose (GLUT) em placentas de gestantes com síndromes hipertensivas da gestação

Abstract

Purpose: to evaluate the expression of CRH, CRH-R1, CRH-R2, GLUT-1 and GLUT-3 in human placentas affected by hypertensive disorders of pregnancy (HDP), and if there are any changes in the expression of these markers in this entity. Methods: retrospective study that evaluated 116 human placenta biopsies between 2012 and 2014. Cases with HDP were classified as preeclampsia (PE), preeclampsia superimposed on chronic hypertension (PSCH), chronic hypertension (CH) and gestational hypertension (GH). Subsequently, cases with HDP were subdivided into term and preterm group. Expression of CRH, CRH-R1, CRH-R1, GLUT-1 and GLUT-3 were evaluated in chorionic villi and decidua through immunohistochemistry. Results: CRH expression was higher in chorionic villi and decidua in the term group of HDP, whereas in decidua of preterm group it was increased in PE and PSCH. CRH-R1 had greater expression in PSCH, CH and GH and lower in PE; in decidua was increased in GH. CRH-R2 had greater expression in chorionic villi only in GH. GLUT-1 expression was greater in chorionic villi in the term group with HDP; in decidua of premature group, its expression was reduced in chronic HDP and in PE. GLUT-3 was increased in PSCH and CH in term group. Conclusion: clinical severity of different HDP favors variations in CRH synthesis. Increased CRH concentration may reduce or exacerbate the expression of CRH-R1 and CRH-R2. The degree and duration of hypoxia seems to regulate the expression of GLUT-1 and GLUT-3 in this entity.