Composição corporal e polimorfismos de deleção dos genes GSTT1 e GSTM1 em síndrome dos ovários policísticos

Abstract

The Polycystic Ovarian Syndrome (PCOS) is considered a multifactorial disorder, with genetic, metabolic, endocrine and environmental alterations, affecting a large part of the female population, being an important factor of infertility. The current information does not yet allow to establish precise parameters for diagnosis and prognosis. Thus, this study aimed to identify risk factors, clinical consequences, anthropometric data, body composition, and deletion polymorphisms of the genes of the glutathione S-transferase GSTT1 and GSTM1 in patients with PCOS, comparing them with a control population . For this, two hundred and forty women (117 patients with PCOS and 123 patients without PCOS) were evaluated. Socio-demographic data, risk factors and clinical consequences were obtained through a questionnaire and follow-up of consultations. Anthropometric data were obtained with the aid of a tape measure and the bioimpedance test used had a tetrapolar system. Insulin resistance tests were performed with insulin, glucose and triglycerides. Molecular analysis was performed from peripheral blood collection and genomic DNA extraction. Genotyping was performed by the multiplex PCR technique. Statistical analysis was performed using the SSP SS, version 24and withlevesignificance was 5% (p≤0.05). The data obtained showed that the absence of gestation, menstrual irregularities, factors associated with infertility and clinical characteristics of hyperandrogenism were more frequent in the group of women with PCOS. In the anthropometric analysis, increased weight and body mass index were associated with PCOS. The indices of insulin resistance also differed between the groups. The frequency of GSTT1 and GSTM1 deletion polymorphisms did not differ between groups. The multivariate analysis showed that the family history of PCOS is more frequent among patients with PCOS. Among the risk factors for the syndrome, the history of familial PCOS was associated with the disease, being frequent in women of the experimental group. To conclude, in our series, family history, clinical consequences of the syndrome, weight, BMI and insulin resistance index are associated with PCOS.