Purificação da Cefalosporina C por cromatografia de troca iônica em coluna de leito fixo

Abstract

This work was to evaluate the feasibility of the process of purification of the antibiotic cephalosporin C (CPC) by ion-exchange chromatography column as stationary phase using the anion exchange resin Amberlite IRA 410. Spite of the use of chromatography be known in ion exchange purification of the CPC process, most of the available literature is in the form of patents, that restricts access to the knowledge of the process as a whole. To evaluate the feasibility of using anion exchange resin Amberlite IRA 410 in the purification process of the CPC experiments were performed using a chromatographic column packed with the resin. In the first step we studied the interaction of CPC with the resin. These experiments showed that the resin is able to adsorb much CPC with well-defined peak and similar adsorption and elution profile at the concentrations studied. The breakthrough curves of CPC were also obtained at three different flow rates. And through some of the same parameters that are important, such as efficient use of the bed, recovery efficiency and product yield were calculated. The results showed that the process has column CPC removal efficiencies above 90% for all flow rates studied. The adsorption of CPC was studied using the resin kinetics and adsorption isotherm at 30°C curve showing the process rapidly reaches equilibrium concentrations studied and obtained data were best fitted to a linear isotherm model. Finally, we tested the possibility to purify a simulated broth containing the CPC and the amino acid methionine, the strategy used for the separation of components was satisfactory and it was possible to promote the separation of CPC and methionine under the conditions studied.