Isolamento biomonitorado de composto antibacteriano produzido por Streptomyces tubercidicus

Abstract

Endophytic microorganisms present a great genetic diversity and are an important source for the discovery of new bioactive secondary metabolites. Natural endophytic products exhibit a broad spectrum of biological activity, including antibiotics, immunosuppressants, anticancer drugs, antioxidants and others.In this study, the endophytic microorganism Streptomyces tubercidicus, isolated from the Solanum lycocarpum St. Hill (Lobeira) plant of the Cerrado of the Universidade Federal de São Carlos (UFSCar),was cultivated and the results demonstrate that the lineage is quite resistant to storage time, as it was isolated almost ten years before the present cultivation. The process of separation and purification of the bioactive extract of S. tubercidicus led to the isolation of only one active fraction. This fraction displays antibacterial activity against Gram + and Gram - bacteria. This result is different from that previously obtained, where two active fractions were obtained, but one with activity against Gram + bacteria and another with activity against Gram - bacteria.The structural elucidation of the bioactive secondary metabolite isolated from S. tubercidicus has not yet been concluded, but it can be concluded that tubercidin has not been isolated as the 1H and 13C NMR spectra of the isolated major compound are distinct from simulated data for tubercidin.