Avaliação do infiltrado tumoral em camundongos induzidos a tumor de mama submetidos à imunoterapia com células dendríticas e atividade física

Abstract

The inflammatory infiltrate has an important participation in the immune response against several diseases, among them cancer. Therefore, the objective of this study was to evaluate the inflammatory infiltrate of mice induced to the breast tumor, treated with the Dendritic Cell vaccine, physical activity and combined therapy (physical activity + dendritic cell vaccine). For the study, 70 BALB / c mice were used, 60 females and 10 of both genera for spinal cord extraction and Dendritic cell vaccine preparation, females were divided into 4 groups with n = 15: untreated tumor group (TTC), tumor group undergoing physical activity (TAF), tumor group treated with dendritic cell vaccine (TDC) and tumor group physical activity plus dendritic cell vaccination (TTC). Tumor of the animals was extracted post euthanasia for (CD4 and CD8), MHC II (IA), costimulatory molecules (CD80, CD86 and CD152) and adhesion molecules (CD54 and CD102) were evaluated by immunofluorescence evaluation of the following markers: helper and cytotoxic T lymphocytes. As results we found a final lower tumor volume statistically significant of the treated groups when compared to the animals of the untreated tumor group, presenting p <0.0030 for the TAF group, p <0.0023 for TDC and p <0.0182 for CT. In the evaluation of the surface markers there were significant differences in MHC II expression in the DC tumor group compared to the T group with p <0.0255. We verified changes in CTLA-4 expression, showing a statistically significant decrease when comparing the BDC group with the TAF and TTC groups, presenting p <0.0178. In relation to the ICAM-1 and ICAM-2 adhesion molecules, we observed a significant increase for ICAM-1 in all groups submitted to some type of immunotherapy, with p <0.0001. The ICAM-2 adhesion molecule demonstrated a significant increase in its expression in the groups that underwent immunotherapeutic intervention. We conclude that the different types of immunotherapy were able to modulate the antitumor immune response causing modifications in the infiltrate that led to a lesser tumor progression.