Fáscia de Scarpa humana: caracterização biomecânica e histológica antes e após a descelularização

Abstract

The Scarpa’s Fascia (SF) can be an additional grafting asset in covering and lift procedures. Our aim was to analyze the histological and biomechanical aspects of the FS prior and after a decellularization process. The in vivo response to fascia the Scarpa was evaluated in mice. FS samples were obtained from patients that went thorough abdominoplasty. The cellularised Scarpa fascia underwent the biomechanical test within the surgical block and decellularized samples underwent the same biomechanical test. The histological slides were prepared for evaluation of collagen and elastin. Cellular and decellularized samples were implanted in the subcutaneous mouse, after 7, 14 and 28 postoperative days were removed and made into slides for analysis of collagen and presence of calcium. There was a decrease in biomechanical properties and an increase and looseness in collagen fibers in the decellularized group. Normal delivery in the cell group decreased the maximum strength of the material, but there was no correlation between the other clinical characteristics of the patients with biomechanical characteristics, but there was correlation of the collagen fibers with the age of the patient. The percentage of collagen was higher on the seventh and fourteen postoperative day compared to the other days. We conclude that there are differences in biomechanical and histological characteristics between cellularized and decellularized fascias, there is no difference between the right and left side and few clinical characteristics such as delivery varginal and age influenced the results.